Education
National Level Exam
DBT-Junior Research Fellowship (DBT-JRF) in Biotechnology through Biotechnology Eligibility Test (BET) | Life Sciences| 2019
Qualified National Institute of Immunology (NII) Ph.D entrance examination three times| Life Sciences| 2019, 2018 & 2017 All India Rank 528 in GATE (Graduate Aptitude Test in Engineering) in Biotechnology| Biotechnology| 2018 All India Rank 68 in Junior Research Fellow category of Council of Scientific and Industrial Research (CSIR)- National Eligibility Test (NET)| Life Sciences| 2017 Qualified JGEEBILS (Dec ), and got selected at TIFR Centre for Interdisciplinary Sciences (TCIS) Hyderabad for PhD| Life Sciences| 2017 All India Rank 32 in Lectureship category of Council of Scientific and Industrial Research (CSIR) National Eligibility Test (NET)| Life Sciences| 2017 All India Rank 1611 in GATE (Graduate Aptitude Test in Engineering) in Life Sciences| Life Science| 2017 All India Rank 41 in M.Sc. Biotechnology IIT JAM (Joint Admission Test For Masters)| Biotechnology| 2015 Scholarship for M.Sc. for the period of 2015-17 sponsored by Department of Biotechnology, Government of India| Biotechnology| 2015-201 |
Degree/ Certification
Doctorate| Neuroscience| 2023| AIIMS, New Delhi Master’s| Biotechnology| 2017| IIT Roorkee Bachelor’s| Zoology (H)| 2015| Sri Venkateswara College, Delhi University |
Area of Research
Molecular Biology, Cell Biology, Biotechnology, Zoology, RNA biology, Biochemistry,
Molecular Biology Gel Electrophoresis, Genomic DNA isolation, RNA isolation, Protein Isolation, Immuno-fluorescence, Immuno-histochemistry, PCR, Real time PCR, Flow cytometry, Western Blotting, Cloning, MTT assay, apoptotic assay, Next generation sequencing.
Cell biology In vitro Disease Modelling, Neural and stem cell culture, Cell culture, tissue culture, stem cell culture, MTT assay, apoptotic assay.
Animal handling- Wistar rats handling, BALB/c mice handling, drug feeding, animal dissection.
Bioinformatics Blast, Primer Designing, Basic Knowledge of RNA sequencing analysis pipeline (tuxedo pipeline), Network analysis, Cytoscape, String, Gene interactions, Target prediction, GSEA, EPD, iDEP, DAVID
Biostatistics Graph pad, Clinical statics, Clinical Data management, Basics of Linux, R operating system
Membership
- Life Member, Indian Academy of Neuroscience (IAN).
- Life Member, Association of Clinical Biochemist of India (ACBICON)
- Member of the Society for Neuroscience (SfN 2023)
Professional Experience
- Aug 2021 – till date Senior Research fellow
- (1 year 10 months) Department of Biochemistry, AIIMS, New Delhi, India
- Principal investigator: Prof. Sudip Sen
- Aug 2019–Sep 2021 Junior Research Fellow
- (2 years) Department of Biochemistry, AIIMS, New Delhi, India
- Principal investigator: Prof. Sudip Sen
- (Dec 2017–July 2018) Junior Research Fellow
- (2 years) Department of Gastroenterology, AIIMS, New Delhi, India
- Principal investigator: Prof. Baibaswata Nayak
Research and Training
- Studying the effect of hypoxia on neurons and oligodendrocytes in vitro – a model to design therapeutic strategies against cerebral palsy in preterm infants.
- Deciphering the osteogenic effect of Symphytum officinale using rodent mode.
- Evaluating the role of JAK-STAT signaling pathway in oligodendrocyte maturation and hypoxic injury in vitro.
- Development of Recombinant Newcastle Disease Virus Model for understanding immune response in patients infected with Hepatitis C Virus.
- Metabolic studies of Indian Medicinal Plant Rutagraveolens for rapid isolation of commercially important phytomedicines from light-irradiated callus.
- Over-expression and purification of NF-kB protein fragmen
Paper Presented at:
- Tyagi Sagar, Rani Sweety, Shrivastava Vadanya, Dey Devanjan, Sen Sudip*. “Elucidating The Role Of TNF-A/JAK-STAT Pathway Axis in A Cell-Line Model of Oligodendrocyte Differentiation In Vitro” presented in 48th National Conference of the Association of Clinical Biochemists of India (ACBICON-2022)
- Tyagi Sagar, Rani Sweety, Shrivastava Vadanya, Dey Devanjan, Seth Pankaj, Sen Sudip*. “Delineating the role of TNF-α & JAK-STAT signaling pathways in promoting oligodendrocyte differentiation in vitro” presented in XL annual meeting of Indian academy of Neuroscience (IAN-2022)
- Rani Sweety, Tyagi Sagar, Shrivastava Vadanya, Dey Devanjan, Seth Pankaj, Sen Sudip* Assessing involvement of the JAK-STAT cell Signalling pathway” in a cell-line model of oligodendrocyte differentiation in vitro. in AIIMS Research Day, AIIMS New Delhi, India
Publication
- Hypoxia Induces Early Neurogenesis in Human Fetal Neural Stem Cells by Activating the WNT Pathway. Mol Neurobiol (2023). https://doi.org/10.1007/s12035-023-03248-4
- Protocol for Developing a Femur Osteotomy Model in Wistar Albino Rats. J. Vis. Exp. (186), e63712, doi:10.3791/63712 (2022).